Objective To screen  the new gene p7TP2  transregulated by HCV p7 protein  through gene chip technique and  investigate  its biological  functions. Methods The p7TP2 cDNA was ampli? ed by PCR and prokaryotic expression vector pET-32a  (＋)-p7TP2 was constructed by gene  recombination  techniques, which were  transformed  into E. coli Rosetta-gami B, for IPTG  induced expression. The p7TP2 fusion protein was expressed  in  the  form of  inclusion body. A band with 35 kD appeared on SDS-PAGE gel. The protein expressed by carboxyl  terminal coding  sequence had a high purity after denaturation,  refolding and purification. New Zealand  rabbits were  immunized with p7TP2  fusion protein  to prepare polyclonal antibody against p7TP2. The p7TP2 antiserun was obtained and characterized by ELISA, Western blot, immunohistochemistry. Results Results  showed  that  the polyclonal antibody had high  titer, affinity and speci? city. Conclusions The study provide a favorable  tool for further functional study of HCV p7TP2 and the pathogenesis of hepatitis C.