Abstract: Objective To testify the transactivated role of HCV NS5A to NS5ATP13 gene and the effect of NS5ATP13 gene expression on Huh-7 cells. Methods RT-PCR was carried out to testify the transactivated role of HCV NS5A to NS5ATP13 gene and NS5ATP13-GFP fusion gene expression vector was constructed. The subcellular localization of NS5ATP13-GFP fusion protein was observed by fluorescence microscope. Results HCV NS5A can transactivate the NS5ATP13 gene expression, NS5ATP13-GFP fusion protein mainly located in the nucleus and most of the cell occured two or more nucleus. Celltiter-Glo luminescent cell viability assay showed that the growth of Huh-7 cells transfected with NS5ATP13 gene was dramatically promoted compared with the parent Huh-7 cells. Conclusions The biological functional study of NS5ATP13 brought some new clues for studying the pathogenesis of HCV induced hepatic fibrosis and hepatoma.