Abstract: Objective To investigate the relationship between the color doppler sonography and contrast-enhanced echocardiography (CEE) in assessment of intrapulmonary shunting in patients with hepatopulmonary syndrome and HIF-1α, MPO and portal hemodynamic. Method The portal vein diameter and blood flow velocity were examined by color doppler, and intrapulmonary shunting by contrast-enhanced echocardiography (CEE). Expressions of HIF-1α and MPO in serum were determined by ELISA in normal, hepatocirrhosis amalgamating HPS and unam algamating HPS patients, separately. HIF-1α and MPO levels were compared among the groups, also the portal hemodynamic and the intrapulmonary shunting were examined. Results The levels of HIF-1α and MPO in hepatocirrhosis amalgamating HPS levels patients were higher than those in unamalgamating HPS levels patients, the diameter of portal vein dilated and the velocity of portal vein dropped, which were statistically different between the two groups (P ＜ 0.05) and positively correlated with intrapulmonary shunting. Conclusions The color doppler sonography and contrast-enhanced echocardiography (CEE) which reflected HIF-1α, MPO levels patients and  the hemodynamic played an role in the development of HPS, and was available to early examine the change of intrapulmonary vessel.