Abstract: Objective To develop a noninvasive diagnosis model of fibrosis based on clinical, serum markers and ultrasonography checking in chronic hepatitis B. Methods Liver biopsy were performed on 374 patients with chronic hepatitis B and staging of fibrosis were assessed. Common clinical indexes, serum markers and ultrasonography checking were detected to develop a diagnosis model of fibrosis. The model was assessed with ROC analysis. It was also applied to the validation group to test its accuracy. Results The model (SZ SZFibroS model) to established fibrosis consisted of platelet count (PLT), albumin/globin (A/G), thickness of spleen, hyaluronic acid (HA) and age. ROC analysis showed that, if SZFibroS ≥ 5.4, the sensitivity of discriminating ≥ S2  was 78.2%, the specificity was 67.7%, and AUC for identifying ≥ S2  was 0.8153. Conclusions The model for assessment of liver fibrosis was simple and repeatable. It could be applied to assist or substitute liver biopsy to detect dynamic changes of liver fibrosis with chronic hepatitis B.