Abstract: Objective To analyze the effect of serum protein electrophoresis (SPE), immunoglobulin and light chain detection in the diagnosis of liver disease. Methods The serum levels of IgG, IgA, IgM, kappa light chain and lambda light chain in 92 patients with liver diseases (28 cases with acute hepatitis, 33 cases with
chronic hepatitis and 31 cases with liver cirrhosis) were detected by turbidimetry and then compared with the indexes in 45 healthy cases. SPE was detected by Helena Spife 3000 electrophoresis apparatus. ResultsSerum IgM level in patients with acute hepatitis was significantly higher than that in control group (P < 0.05).
Kappa light chain and lambda light chain showed no significant difference between acute hepatitis group and control group. The serum levels of IgG, IgA, kappa light chain and lambda light chain in patients with chronic hepatitis and liver cirrhosis were significantly higher than that in control group (P < 0.05). SPE results
showed that the level of γ globulin in patients with chronic hepatitis and liver cirrhosis were significantly higher than that in controls (P < 0.05). Conclusions The high level of immunoglobulin and light chain are related to liver disease. Serum protein electrophoresis, immunoglobulin and light chain detection have high value in laboratory monitoring of liver disease.
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