Abstract: Objective To analyze the influence of polyene phosphatidyl choline (PPC) on liver fibrosis and cytokines in patients with chronic hepatitis B (CHB). Methods Total of 98 cases with CHB were randomly divided into the treatment group (n = 50) and control group (n = 48). The serum levels of transforming growth factor-β1  (TGF-β1 ), tumor necrosis factor-α (TNF-α), hyaline acid (HA), collagen type Ⅳ (C-Ⅳ), laminin (LN), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in those patients were detected before and after treatment. Results After treatment, the serum levels of TGF-β1 , TNF-α, ALT, AST, HA, C-Ⅳand LN in treatment group were significantly reduced, and lower than those in control group (P ＜ 0.01). Conclusions PPC has strong antioxidant, cytoprotective, anti-inflammatory and anti-fibrotic effects.