Abstract: Objective To assess the credibility of liver biopsy which is considered to be a gold standard for fibrosis evaluation. Methods The pathological sections (silver staining, HE staining, immunohistochemistry) of 125 patients with chronic hepatitis B were collected. These sections were single-blind read by the other pathologist. All results were compared and analyzed. Results Total of 123 pathological sections of different hepatic histological inflammation (G) were collected, with coincidence rates of G0 to G4 are 16.67%, 25.80%, 21.05%, 53.85% and 25.00%, respectively, there’s significant difference (χ2 = 58.352, P = 0.000); 125 pathological sections of different liver fibrosis (S) stages were collected, and coincidence rates of S0 to S4 were 72.70%, 17.40%, 65.50%, 54.84% and 45.16%, respectively, with significant difference (χ2 = 15.451, P = 0.004); 117 pathological sections of different distribution types of HBcAg were collected, coincidence rates of HBcAg negative, cytoplasm distribution, nuclei distribution and mixed type distribution were 77.42%, 42.10%, 75.00% and 6.78%, respectively, with significant difference (χ2 = 68.238, P = 0.000). Conclusions There’s significant differences among the results given by different pathological professors viewing the same liver biopsy from patients with chronic hepatitis B, especially in assessing liver biopsy of G2 or G3 and S1 or S2. Thc assessment of liver biopsy should be made in combination with other clinical data.