Abstract: Objective To screening the differential gene expression in human hepatic stellate cells (HSC) treated with pcDNA3.1-ALR in order to investigate the biological functions and mechanism of human augmenter of liver regeneration (hALR). Methods HSC were transfected by pcDNA3.1 (－) and pcDNA3.1-ALR, respectively, using FuGENE6 transfection reagent. The total mRNA was isolated and reverse transcribed to synthesize the cDNA probes. Analyzed the differentially expressed genes by microarray gene expression profiling technique. Results Two genes were up-regulated and 25 genes were down-regulated among detected 4096 genes. Conclusions hALR has marked effect on gene expression profilings of human HSC. These results provide new clues for studying the molecular biological function and mechanism of hALR.