Abstract: Objective To establish an appropriate murine model for this study, the mice were administrated by single intraperitoneal injection at different doses with carbon tetrachloride (CCl4) to induce acute liver injury, and the changes of aminotransferase levels in plasma were measured. Methods Acute liver injury in C57BL/6 male mice (6-8 weeks) were administrated by single intraperitoneal injection with CCl4. Comparing the aminotransferase levels in plasma from different doses of CCl4-injected mice. The 32 mice were randomly separated from 182 mice. The 32 mice were randomly divided into normal control group (8 mice) and 0.1%, 0.2% and 0.3% CCl4 groups (8 mice per group) which received 0.1%, 0.2% and 0.3% CCl4 (10 ml/kg, dissolved in olive oil) respectively, while the normal control group received only olive oil as control. Twelve hours after challenge, the mice were sacrificed and plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured. Comparing the aminotransferase levels in plasma from 0.1% and 0.2% groups at different time points. The 150 mice were randomly divided into normal control group (8 mice), the experimental group Ⅰ (0.1% dose, 70 mice) and the experimental group Ⅱ (0.2% dose, 70 mice). Plasma levels of ALT and AST were measured at 6, 12, 16, 20, 24, 48 and 72 hours after challenge. Results Compared with normal control group, plasma ALT and AST were significantly increased (P ＜ 0.05) in CCl4-treated mice at 12 hour, furthermore, the effect was dose-dependent in 0.1%, 0.2% and 0.3% groups. The levels of ALT and AST rised at the early phase in 0.1% and 0.2% groups, and reached the peak at 16 and 20 hours, respectively. Then the levels declined gradually and were close to the normal level at 72 hours. Conclusions The increase of ALT and AST levels was in a dose-dependent manner with CCl4 by single intraperitoneal injection. To establish acute mild liver injury in C57BL/6 mice, 0.1% of CCl4 was the appropriate dose, and 16 hours after challenge was the suitable time point for detection of aminotransferase levels.