Abstract: Objective To realize the risk of HBV infection in the public service places in Karamay and to provide a scientific basis for hepatitis B prevention and control. Methods There are 12 beauty parlors, barber shops and pedicure shops which were opened all year round or relatively fixed by random sampling method. There are 252 workers whose serum were collected were investigated by questionnaire and the appliances in the public service places before using and after using were collected. HBsAg from the appliances of the public service places and workers were detected by MEIA. Results The positive rate of HBsAg among the workers in the small-sized public service places was 11.76%, significantly higher than the workers in the large and medium-sized public service places. Appliances in the public service places did not detect the HBsAg before using, but the rates of testing on HBsAg among the appliances after using were 1.22% and were 5.56% in the small-sized public service places. The positive rate of HBsAg on the appliances of beauty parlor was 1.27%. Conclusions Appliances used in the public service places were detected HBsAg positive in the study. We should enhance the sanitizing and using management of the appliances used in the public service places and the knowledge of control and prevention of hepatitis B. Utmost to prevent the spread of hepatitis B in places of public service.