Abstract: Objective To investigate the expression of plasma homocysteine levels and liver function index (ALT, AST, TBil, CHE) in patients with hepatitis B. Methods We using electrochemical luminescence instrument and automatic biochemical analyzer for hepatitis B patients and healthy controls of plasma homocysteine levels and liver function index (ALT, AST, TBil, CHE) for testing analysis. Results Plasma homocysteine levels in patients with hepatitis B was significantly higher than the control group. The change was positively correlated with ALT, AST, TBil was negatively correlated with CHE. Conclusions Serum levels of homocysteine is an indicator of disease detection in patients with hepatitis have clinical value for disease diagnosis.