中国肝脏病杂志(电子版)

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脂易消对NAFLD大鼠Visfatin表达的影响

作者：周岳君

单位：浙江中医药大学杭州 310053

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出版年,卷(期):页码：2015,7(3):52-55

摘要：

目的观察脂易消对实验性非酒精性脂肪性肝病（non-alcoholic fatty liver disease，NAFLD）大鼠肝组织内肥素（visfatin）的影响，探讨脂易消治疗NAFLD的作用机制。方法采用高脂饮食制备NAFLD大鼠模型。大鼠分低、中、高剂量脂易消治疗组、易善复阳性对照组、生理盐水阴性对照组、正常对照组。采用RT-PCR法检测大鼠肝脏Visfatin mRNA的表达水平，ELISA法检测大鼠肝组织Visfatin蛋白的表达水平。结果脂易消各治疗组大鼠肝组织Visfatin蛋白表达与Visfatin mRNA表达均明显降低（F = 19.9，P = 0.001；F = 36.1，P = 0.001）；但与易善复组相比，脂易消各治疗组大鼠肝组织Visfatin蛋白表达和脂易消中、高剂量组大鼠肝组织Visfatin mRNA表达均无显著性差异（P分别为0.990、0.710、0.864、0.993、0.113），脂易消低剂量组与易善复组肝组织Visfatin mRNA表达水平存在统计学差异（P = 0.006）。结论脂易消能调控肝组织Visfatin表达，降低肝组织Visfatin蛋白表达和Visfatin mRNA表达水平，其疗效与易善复相似；其治疗NAFLD机理可能与脂易消调控肝组织Visfatin表达水平有关。

Abstract: Objective To investigate the effect of Zhiyixiao on the Visfatin of non-alcoholic fatty liver disease (NAFLD) rat in liver tissue and to discuss the mechanism of Zhiyixiao in treatment of NAFLD. Methods High-fat diet feeding NAFLD rats were divided into Zhiyixiao treatment group, Yishanfu treatment group, saline negative group and the normal control group. Each group was furtherly diveded into three dosage subgroups. Vasfatin mRNA in liver tissue was detected by PT-PCR, and Visfatin protein in liver tissue was measured by ELISA. Results The Visfatin protein and mRNA of Zhiyixiao treatment group were significantly lower in liver tissue (F = 19.9, P = 0.001; F = 36.1, P = 0.001) compared with Yishanfu group, each dose group had no significant difference (P = 0.990, 0.710, 0.864, 0.993, 0.113 respectively). Conclusions Zhiyixiao can reduce the expression of Visfatin protein and mRNA in liver tissue, whose efficacy is similar to Yishanfu, and the mechanism may relate to the regulation of Zhiyixiao on Visfatin expression in liver tissue.

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