中国肝脏病杂志(电子版)

位置：首页 >> 期刊文章

绞股蓝皂苷和银杏叶提取物混合物对2型糖尿病并NAFLD大鼠血瘦素、脂联素和肝组织丙二醛、超氧化物歧化酶的影响

作者：赵琴1 2 李儒贵 1 李金科 1 李芳 1 李刚 1 谭华炳 1

单位：1. 湖北医药学院附属人民医院感染性疾病科肝病研究所湖北十堰 442000 2. 湖北省房县人民医院检验科湖北房县 442100

关键词：绞股蓝皂苷银杏叶提取物 2型糖尿病脂肪肝非酒精性瘦素脂联素丙二醛超氧化物歧化酶

分类号：

出版年,卷(期):页码：2016,8(1):62-66

摘要：

摘要：目的观察绞股蓝皂苷（gypenosides，GPS）和银杏叶提取物（ginkgo biloba extract，GBE）
混合物对2型糖尿病（type 2 diabetes mellitus，T2DM）并非酒精性脂肪性肝病（non-alcoholic fatty
liver disease，NAFLD）大鼠血浆瘦素（leptin，LP）、脂联素（adiponectin，APN）和肝组织甘油三
酯（TG）沉积量、丙二醛（malondialdehyde，MDA）、超氧化物歧化酶（superoxide dismutase，
SOD）的影响。方法 40只SPF级雄性SD大鼠（体质量为220～250 g）随机分为空白对照组（空白组，
n = 7），T2DM并NAFLD模型组（n = 33）。造模周期8周。将T2DM并NAFLD模型大鼠随机分为GPS
治疗对照组[简称对照组，给予GPS 0.5 g/(kg·d)灌胃，n = 10]，GPS和GBE混合物治疗组[简称治疗组，
给予GPS 0.5 g/(kg·d)联合GBE 0.1 g/(kg·d)，n = 10]，模型对照组（简称模型组，给予同等体积的纯净
水灌胃，n = 10）。继续给予高糖高脂饲料，自由饮水，治疗周期为6周，实验共计14周。检测各组
LP、APN、肝组织TG、MDA、SOD水平。结果 ①肝组织MDA变化：空白组（3.01 ± 0.10）nmol/ml、
模型组（4.78 ± 0.12）nmol/ml、治疗组（3.60 ± 0.09）nmol/ml、对照组（4.29 ± 0.11）nmol/ml，治疗组
低于对照组（t = 15.35，P = 0.000）。②肝组织SOD变化：空白组（268.4 ± 15.2）U/ml、模型组（140.6 ±
16.8）U/ml、治疗组（224.8 ± 15.4）U/ml、对照组（180.6 ± 16.0）U/ml，治疗组低于对照组（t = 6.29，
P = 0.000）。③血APN变化：空白组（7.98 ± 0.96）ng/ml、模型组（4.01 ± 0.88）ng/ml、治疗组（5.92 ±
0.12）ng/ml、对照组（5.11 ± 0.10）ng/ml，治疗组低于对照组（t = 16.40，P = 0.000）。④血LP变化：
空白组（2.82 ± 0.58）ng/ml、模型组（7.89 ± 0.68）ng/ml、治疗组（4.68 ± 0.86）ng/ml、对照组（5.89 ±
0.76）ng/ml，治疗组低于对照组（t = 3.73，P = 0.004）。⑤肝组织TG：空白组（30.26 ± 2.48）mg/g、
模型组（228.46 ± 8.48）mg/g、治疗组（153.12 ± 9.98）mg/g、对照组（196.24 ± 9.78）mg/g，治疗组低
于对照组（t = 9.76，P = 0.000）。结论 GPS和GBE混合物通过抑制脂质过氧化，调整LP/APN平衡，以
减少TG在肝组织的沉积。

Abstract: Objective To observe the effects of gypenosides (GPS) and ginkgo biloba extract (GBE) mixture
on plasma leptin (LP), adiponectin (APN), hepatic tissue malondialdehyde (MDA) and superoxide dismutase
(SOD) in rats with type 2 diabetes mellitus (T2DM) and non-alcoholic fatty liver disease (NAFLD). Methods
Total of 40 SPF male SD rats (body mass 220～250 g) were randomly divided into blank control group
(n = 7) and model group with T2DM and NAFLD (n = 33). After 8 weeks, the NAFLD model rats were
prepared. The model group was then divided into three subgroups: the GPS and GBE mixture intervention
group (treatrment group, n = 10) were perfused with 0.5 g/(kg·d) GPS and 0.1 g/(kg·d) GBE mixture, the GPS
intervention group (control group, n = 10) were perfused with 0.5 g/(kg·d) GPS, and the model group (n = 10)
were perfused with the same volume of water. All the groups were given high fat diet and free drinking, the
treatment course was 6 weeks and the experimental period was 14 weeks. The levels of LP and APN in plasma
and the MDA and SOD in hepatic tissue were detected. Results ①The hepatic tissue MDA level in blank group,
model group, treatment group and control group were (3.01 ± 0.10) nmol/ml, (4.78 ± 0.12) nmol/ml, (3.60 ±
0.09) nmol/ml and (4.29 ± 0.11) nmol/ml, respectively. The MDA level in treatment group was lower than that
of the control group, with a significant difference (t = 15.35, P = 0.000). ②The hepatic tissue SOD level in the
blank group, model group, treatment group and control group were (268.4 ± 15.2) U/ml, (140.6 ± 16.8) U/ml,
(224.8±15.4) U/ml, and (180.6 ± 16.0) U/ml, respectively. The SOD level in the treatment group was lower than
that of the control group, with a significant difference (t = 6.29, P = 0.000). ③The plasma APN level in the blank
group, model group, treatment group and control group were (7.98 ± 0.96) ng/ml, (4.01 ± 0.88) ng/ml, (5.92 ±
0.12) ng/ml, and (5.11 ± 0.10) ng/ml, respectively. The APN level in treatment group was lower than that of
the control group, with a significant difference (t = 16.40, P = 0.000). ④The plasma LP levels in the blank
group, model group, treatment group and control group were (2.82 ± 0.58) ng/ml, (7.89 ± 0.68) ng/ml, (4.68 ±
0.86) ng/ml, and (5.89 ± 0.76) ng/ml, respectively. The LP level in the treatment group was lower than that of
the control group, with a significant difference (t = 3.73, P = 0.004). ⑤The plasma TG levels in the blank
group, model group, treatment group and control group were (30.26 ± 2.48) ng/ml, (228.46 ± 8.48) ng/ml,
(153.12 ± 9.98) ng/ml, and (196.24 ± 9.78) ng/ml, respectively. The TG level in the treatment group was
lower than that of the control group, with a significant difference (t = 9.76, P = 0.000). Conclusion The
reduction of TG in the liver tissue deposition of rats with T2DM and NAFLD was meddled by GPS and GBE
mixture through inhibiting the lipid peroxidation and adjusting the LP/APN balance.

基金项目：

作者简介：

参考文献：

服务与反馈：

【文章下载】【加入收藏】