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Glypican-3对HepG2肝癌细胞增殖及侵袭转移的影响

作者：张韬 1 朱琪 2 程变巧 2 林伟国 2

单位：1.厦门大学附属福州第二医院中心实验室福州 350007 2.厦门大学附属福州第二医院肝胆内科福州 350007

关键词：Glypican-3 肝肿瘤增殖侵袭转移

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出版年,卷(期):页码：2016,8(2):63-67

摘要：

摘要：目的探讨磷脂酰肌醇蛋白多糖-3（Glypican-3，GPC-3）在HepG2肝癌细胞增殖及侵袭转移中
的作用。方法构建并筛选沉默GPC-3最有效的shRNA，转染HepG2细胞，Western blot分析GPC-3蛋白
表达水平；MTT、划痕实验和Transwell小室实验分别检测GPC-3基因沉默对HepG2细胞增殖、转移和
侵袭能力的影响。结果 GPC-3-shRNA-1干扰效果最好，对GPC-3表达抑制率为77.7%；GPC-3沉默后
HepG2细胞增殖显著受到抑制（P = 0.014），细胞转移和侵袭能力显著减弱，侵袭抑制率为52.4%。
结论 GPC-3基因沉默后HepG2细胞增殖、转移和侵袭能力显著受抑制，提示GPC-3在肝癌的发生发展
中起关键作用，可能成为肝癌靶向治疗的目标。

Abstract: Objective To investigate the effects of Glypican-3 on proliferation, invasiveness and migratory
metastasis of HepG2 cells. Methods High effective GPC-3-shRNA was obtained by construction and screening.
The expression of GPC-3 was analyzed by western blot after transfected into HepG2 cells. The effects of gene
silence of GPC-3 on proliferation, invasiveness and migratory metastasis of HepG2 cells were analyzed by
MTT, cell scratch test and transwell chamber system, respectively. Results The most effective shRNA inhibited
77.7% expression of GPC-3. The proliferation of HepG2 cells were significantly inhibited after GPC-3 silence
(P = 0.014). The migratory metastasis and invasiveness with reduced significantly, and the invasion inhibition
rate was 52.4%. Conclusion The proliferation, invasiveness and migratory metastasis of HepG2 cells were
significantly inhibited after GPC-3 silence, which suggests that GPC-3 may play an important role in the
development of hepatic carcinoma and contribute to targeted therapy for hepatic carcinoma.

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