Abstract: Objective To construct the shRNA interference lentivirus vector for Akt2 and evaluate its effects
on human HepG2.2.15 cell mediated by lentivirus. Methods RNAi sequences for Akt2 were designed using
bioinformatics methods. Lentiviral vectors, expressed in E.coli and packaged by 293T cells, were used to
construct the Akt2 shRNA vectors. Dilution method was applied to measure the transfection efficiency and
titer according to the green fluorescent protein (GFP) tracer. Real-time fluorescence quantitative method were
used to measure the interference effects of target sequences. Results Three Akt2 targeting sequences were
constructed and their corresponding shRNA lentiviral vectors were screened for efficiency. One Akt2 shRNA
lentiviral vector was screened by transient transfection (interference efficiency reached 85%) as the best
efficiency and its working condition was established as well. Conclusion Akt2 shRNA lentiviral vectors was
successfully constructed and screened, which can inhibit the expression of Akt2 in human HepG2.2.15 cell
effectively.