Abstract: Objective To observe the effects of NS5ATP9 on cell apoptosis of HepG2 cells changes with
the different starvation time. Methods NS5ATP9 expression vector, small molecules of NS5ATP9 siRNA
interference and their respective control were transiently transfected into HepG2 cells for 48 hours,
respectively. The cells were then starvated with EBSS for 12, 24, 36 and 48 hours. Part cells were used to
observe cell apoptosis changes staining by Annexin V / 7-AAD. Part cells were used to detected caspase-3/7
activities. Results ①Compared with control group, the apoptosis rate of HepG2 in NS5ATP9 overexpressed
group reduced at 24, 36 and 48 hours (t = 17.132, 9.138, 17.318; P = 0.003, 012, 0.003), and the caspase-3/7
activities reduced at 12, 36 and 48 hours (t = 7.637, 6.944, 13.490; P = 0.017, 0.02, 0.005). The differences
were most significant at 48 hours. ②Compared with control group, the apoptosis rate of HepG2 in si-
NS5APT9 transfected group increased at 36 and 48 hours (t = -5.064, -4.342; P = 0.034, 0.049), and the
caspase-3/7 activities increased at 12, 36, and 48 hours (t = -8.837, -7.469, -18.630; P = 0.013, 0.017, 0.003).
The differences were most significant at 48 hours. Conclusion NS5ATP9 in HepG2 cells can significantly
inhibit the apoptosis induced by starvation; with the starvated time prolonged, the effect of NS5ATP9
inhibiting cell apoptosis becomes more obvious and the difference was most significant at 48 hours.