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急性胆道感染对大鼠肝细胞凋亡相关蛋白表达的影响

作者：李娜 1 伊秀梅 2 张磊 1

单位：1.淄博市中心医院消化内一科山东淄博 255000 2.淄博市中心医院门诊山东淄博 255000

关键词：胆道感染急性肝细胞凋亡感染模型

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出版年,卷(期):页码：2017,9(1):87-90

摘要：

摘要：目的研究急性胆道感染对大鼠肝细胞凋亡相关蛋白表达的影响。方法选取实验大鼠35只，适
应喂养1周后将其随机分为5组，每组7只。实验组大鼠均于胆总管注入大肠埃希菌0.2 ml，诱导急性胆
道感染模型，分别于注入6小时、12小时、24小时和48小时后处死；对照组大鼠胆总管内注入生理盐
水，6小时后处死。留取各组大鼠肝组织，采用Tunel染色法统计凋亡肝细胞计数，采用免疫组织化学
法计算肝脏Bcl-2与Bax阳性表达率。结果对照组肝细胞病理无明显改变，凋亡肝细胞少见；各实验
组均可见肝细胞坏死、变形，并发生凋亡。对照组凋亡指数显著低于感染6小时、12小时、24小时和
48小时组，差异有统计学意义（t值分别为5.921、7.559、8.919、10.520，P均＜ 0.001）。对照组和感
染6小时组Bcl-2阳性表达率分别为85.71%和71.43%，差异无统计学意义（χ 2 = 0.000，P = 1.000）；感
染6小时和12小时组的Bcl-2阳性表达率显著高于感染12小时和24小时组（P均＜ 0.05）。对照组、感
染6小时、12小时、24小时和48小时组的Bax阳性表达率分别为14.29%、28.57%、42.86%、100.00%和
85.71%，组间差异有统计学意义（F = 9.314，P ＜ 0.001），Bax阳性表达率随感染时间延长呈升高趋
势，于感染48小时有所下降。结论急性胆道感染短时间即可诱导肝细胞凋亡，并伴有凋亡相关蛋白
Bcl-2阳性表达率的下降和Bax阳性表达率的升高。

Abstract: Objective To investigate the influence of acute biliary tract infection on apoptosis-related protein expression
of liver cells in rats. Methods Total of 35 rats were selected and randomly divided into five groups after adaptive
feeding for 1 week, 7 rats in each group. Rats in experimental groups were given injection of Escherichia coli into
common bile duct to make acute biliary tract infection model and sacrificed after 6 h, 12 h, 24 h and 48 h of injection,
respectively. Rats in control group were injected with normal saline into the common bile duct and sacrificed after 6
hours of injection. The number of apoptotic liver cells was counted by Tunel staining and positive expression rate of
Bcl-2 and Bax in liver were detected by immunohistochemistry method. Results The liver cells in control group had
no obvious pathological changes and the apoptotic hepatocytes were rare. The liver cells in all experimental groups
were found necrotic and deformed and occured apoptosis. The apoptosis index (AI) in control group were significantly
lower than those at 6 h, 12 h, 24 h and 48 h after infection (t = 5.921, 7.559, 8.919, 10.520; P ＜ 0.001). The positive
expression rate of Bcl-2 in control group and 6 h infection group were 85.71% and 71.43%, respectively, the difference
had no statistical significance (χ 2 = 0.000, P = 1.000). The positive expression rate of Bcl-2 in 6 h and 12 h infection
groups were significantly higher than those of 12 h and 24 h infection group (P ＜ 0.05). The positive expression
rate of Bax in control group, 6 h, 12 h, 24 h and 48 h infection group were 14.29%, 28.57%, 42.86%, 100.00% and
85.71%, respectively, the difference was statistically significant (F = 9.314, P ＜ 0.001). The positive expression rate
of Bax increased with time going on, and decreased in 48 h after infection. Conclusion Acute biliary tract infection
can induce the apoptosis of liver cells in a short time, which is accompanied by decreased expression of apoptosis
related protein Bcl-2 and increased expression of Bax.

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