Abstract: Objective To investigate the relationship among hepatitis B surface antigen (HBsAg) quantitation,
hyaluronic acid (HA) and Ⅲ procollagen (PCⅢ) in order to provide a new model parameter for the
establishment of a more accurate noninvasive hepatic fibrosis model. Methods Total of 100 cases with
chronic hepatitis B (CHB) and 60 chronic HBV carriers from June 2015 to April 2016 in our hospital were
selected and the levels of HBsAg, HA and PCⅢ were detected. The levels of HA and PCⅢ of CHB patients
with different HBsAg quantitative values were compared and the relationship among HBsAg, HA and
PCⅢ were analyzed. The levels of HA and PCⅢ between patients with CHB and chronic HBV carriers
and those between serum and plasma were also compared. Results For CHB patients with different HBsAg
quantitative values (HBsAg ＜ 1500 IU/ml and HBsAg ≥ 1500 IU/ml), there were no correlation between
HBsAg quantitative values and the levels of HA and PCⅢ (P ＞ 0.05). The levels of HA and PCⅢ in serum
and plasma were statistically different (t = 4.790, P ＜ 0.001; t = 20541, P = 0.020). The levels of HA and
PCⅢ also had statistically significant differences between patients with CHB and chronic HBV carriers (Z =
-2.323, P = 0.020, t = -8.201, P ＜ 0.001). Conclusions There is no correlation between HBsAg quantitative
values and the levels of HA and PCⅢ in static condition of patients with CHB. The content of HA and PCⅢ
in patients with CHB are significantly higher than those of chronic HBV carriers. The specimens should be
unified when detecting the levels of HBsAg, HA and PCⅢ.