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<p>Abstract: Objective To investigate the effects of deletion of unknown function gene C6orf120 on the<br /> activation of CD4 + T cells in autoimmune hepatitis rats. Methods Wild type and C6orf120 knockout rates<br /> were randomly divided into 5 groups, 8 rats in each group. Rates were given Con A 30 mg/kg by intravenous<br /> injection to establish autoimmune hepatitis model and sacrificed at 0 h, 12 h, 24 h, 48 h and 72 h before and<br /> after Con A challenging. The organs were weighed to calculate the index of immune organs. The CD4 + T cell<br /> activation in splenocytes, peripheral blood mononuclear cells and intrahepatic lymphocytes were analyzed<br /> by flow cytometry. Meanwhile, the ratio of CD4 + /CD8 + cells in peripheral blood mononuclear cells was<br /> evaluated. Results The thymus index of C6orf120 knockout rats decreased significantly (F = 20.868，P ＜<br /> 0.001). C6orf120 deficiency increased CD4 + T cell activation in splenocytes in 12 h and 24 h (t = 3.538, 2.547;<br /> P = 0.003, 0.023). Meanwhile, C6orf120 deficiency increased CD4 + T cell activation in peripheral blood and<br /> intrahepatic lymphocytes (F = 33.801, 55.015; P ＜ 0.001). In addition, C6orf120 deficiency significantly</p> <p>upregulated the CD4 + /CD8 + ratio (F = 55.989, P ＜ 0.001). Conclusion C6orf120 deficiency can upregulate<br /> the activation of CD4 + T cells in autoimmune hepatitis rats.</p>
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