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摘要：
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摘要：目的 探讨三种药物对X射线损伤后肝实质细胞增殖的影响。方法 将人肝实质HL-7702细胞株分为
5组：异甘草酸镁组（照射前加入异甘草酸镁注射液）、还原型谷胱甘肽组（照射前加入注射用还原型
谷胱甘肽）、多烯磷脂酰胆碱组（照射前加入多烯磷脂酰胆碱注射液）、对照组（单纯照射）和非照射
组（正常细胞）。使用6 MV X射线照射细胞株，采用倒置显微镜观察各组照射24 h、48 h及72 h后的
细胞形态，使用四甲基偶氮唑盐（MTT）法检测细胞存活率，分析多烯磷脂酰胆碱注射液、还原型谷
胱甘肽注射液及异甘草酸镁注射液对受损肝细胞增殖修复的影响。结果 倒置显微镜观察示非照射组细
胞呈团状分布，分裂快，细胞为多角形，体积较小，对照组细胞生长受到显著抑制，细胞核变大，细
胞边缘不清晰，体积变大且肿胀，细胞增殖变慢；细胞活力检测示照射24 h时肝细胞受抑制不显著，
主要是细胞形态发生变化，但照射48 h及72 h时对照组细胞吸光度较非照射组显著降低，差异有统计学
意义（P ＜ 0.05）。三种吸光度最高的药物浓度下，异甘草酸镁（1.0 mg/ml）对细胞增殖的有效率为
38.60%，多烯磷脂酰胆碱（250 mol/L）为11.58%，GSH（0.1 mg/ml）未显示促增殖作用。照射24 h、
48 h及72 h时，1.0 mg/ml异甘草酸镁组、250 mol/L多烯磷脂酰胆碱组的细胞A值均高于对照组及GSH组
（P ＜ 0.05），24 h、48 h时1.0 mg/ml异甘草酸镁组和250 mol/L多烯磷脂酰胆碱组MDA含量低于对照
组及GSH组，48 h时细胞SOD活力高于对照组及GSH组，差异均有统计学意义（P ＜ 0.05）。结论 异
甘草酸镁对X射线照射后人肝实质细胞增殖修复显著优于多烯磷脂酰胆碱及GSH，对放射性肝损伤治
疗前景广阔，但具体作用机制仍需更深入研究。
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Abstract: Objective To investigate the effects of three drugs on the proliferation of hepatocytes after X-ray
injury. Methods Human liver parenchyma HL-7702 cell lines were divided into 5 groups: magnesium
isoglycyrrhizinate group (adding magnesium isoglycyrrhizinate injection before irradiation), reduced
glutathione group (adding reduced glutathione before irradiation), polyene phosphatidylcholine group
(adding polyene phosphatidylcholine before irradiation), control group (simple irradiation) and non-irradiated
group (normal cells). The cells were irradiated with 6 MV X-raysmorphology and was observed by inverted
microscope at 24 h, 48 h and 72 h after irradiation. The cell viability was measured by MTT assay. The effects
of polyene phosphatidylcholine injection, reduced glutathione injection and magnesium isoglycyrrhizinate
injection on proliferation and repairment of injured hepatocytes were analyzed. Results Inverted microscope
observation showed that the non-irradiated group showed a cluster-like distribution, the division was fast, the
cells were polygonal, and the volume was small. The growth of the control group was significantly inhibited,
the nucleus became larger, the cell edge was unclear, the volume became larger and swollen, and the cell
proliferation Slow down. The cell viability assay showed that the inhibition of hepatocytes was not significant
at 24 h after irradiation, mainly due to the change of cell morphology. However, the absorbance of the control
group was significantly lower than that of the non-irradiated group at 48 h and 72 h after irradiation (P ＜ 0.05).
The effects of magnesium isoglycyrrhizinate (1.0 mg/ml) and polyene phosphatidylcholine (250 mol/L) on
cell proliferation were 38.60% and 11.58%, respectively. GSH (0.1 mg/ml) showed no effect of promoting
proliferation. At 24 h, 48 h and 72 h after irradiation, the A values of magnesium isoglycyrrhizinate group
(1.0 mg/ml) and polyene phosphatidylcholine group (250 mol/L) were higher than those of control group
and GSH group. The content of MDA in magnesium isoglycyrrhizinate group (1.0 mg/ml) and polyene
phosphatidylcholine group (250 mol/L) were lower than thoset in control group and GSH group at 24 h and
48 h after irradiation, and the cell SOD activity at 48 h was significantly higher than that in control group and
GSH group (P ＜ 0.05). Conclusions Magnesium glycyrrhizinate is superior to polyene phosphatidylcholine
and GSH in the proliferation of human hepatocytes after X-ray irradiation. It has a broad prospect for the
treatment of radiation-induced liver injury, but the specific mechanism still needs further study.
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