|
摘要：
|
|
摘要：目的 探讨维生素K 2 对大鼠HepG2细胞侵袭和凋亡的影响及机制。方法 选取对数生长期的
HepG2细胞，培养48 h后加入不同浓度（1 μmol/L、10 μmol/L、20 μmol/L）的维生素K 2 ，同时设
置对照组（仅加培养液），培养48 h后检测HepG2细胞的增殖、侵袭和凋亡以及肝癌衍生生长因子
（hepatoma derived growth factor，HDGF）的表达。结果 维生素K 2 呈现剂量依赖性抑制HepG2细胞
增殖、增加细胞凋亡指数并抑制HepG2细胞的穿透能力，与对照组相比差异均有统计学意义（P均＜
0.05）。维生素K 2 可呈现剂量依赖性抑制HDGF mRNA与蛋白表达水平，实验组表达水平显著低于对
照组（F = 20.332，P ＜ 0.001）。结论 维生素K 2 可抑制大鼠HepG2细胞的增殖、侵袭并促进凋亡，其
作用机制可能与抑制HDGF基因与蛋白表达有关。
|
|
Abstract: Objective To investigate the effects and mechanisms of vitamin K 2 on the invasion and apoptosis
of HepG2 cells in rats. Methods HepG2 cells in logarithmic growth phase were selected and cultured for
48 hours, then vitamin K 2 of different concentrations (1 μmol/L, 10 μmol/L and 20 μmol/L) were added.
The control group was also set up (only added culture solution). The proliferation, invasion and apoptosis of
HepG2 cells and the expression of hepatoma derived growth factor (HDGF) were detected after 48 hours’
culture. Results Vitamin K 2 inhibited the proliferation, increased the apoptosis index and inhibited the
penetrability of HepG2 cells in a dose-dependent manner. Compared with the control group, the differences
were statistically significant (P ＜ 0.05). Vitamin K 2 inhibited the expression of HDGF mRNA and protein
in a dose-dependent manner, which was significantly lower than that of the control group (F = 20.332，P ＜
0.001). Conclusions Vitamin K 2 can inhibit the proliferation, invasion and enhance the apoptosis of HepG2
cells in rats, and the mechanism may be related to the inhibition of HDGF gene and protein expression.
|
|
基金项目：
|
|
|
|
作者简介：
|
|
|
|
参考文献：
|
|
|
|
服务与反馈：
|
|
【文章下载】【加入收藏】
|
|
|
|