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摘要：
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摘要：目的 分析慢性乙型肝炎（chronic hepatitis C，CHB）患者外周血单个核细胞（peripheral
blood mononuclear cells，PBMC）中miR-155和细胞因子信号转导抑制分子1（suppressor of cytokine
signaling 1，SOCS1）的表达水平，并探讨其与肝功能损伤程度的相关性。方法 连续纳入2016年
3月至2018年6月于南京中医药大学附属南京医院就诊的95例CHB患者为研究对象（CHB组），根
据患者肝组织病理学检查结果分为轻度CHB组（31例）、中度CHB组（38例）和重度CHB组（26
例）。另选取同期健康体检者50例作为对照组。采用RT-PCR检测受试者外周血PBMC中miR-155
和SOCS1 mRNA相对表达量，ELISA法检测CHB患者血清肝功能指标 [丙氨酸氨基转移酶（alanine
transaminase，ALT）、天门冬氨酸氨基转移酶（aspartate aminotransferase，AST）和总胆红素（total
bilirubin，TBil）]，分析外周血miR-155和SOCS1 mRNA相对表达量与CHB严重程度及肝功能指标
的相关性。结果 CHB组患者miR-155相对表达量（0.64 ± 0.15）显著低于对照组（0.86 ± 0.27），
SOCS1 mRNA相对表达量（1.25 ± 0.37）显著高于对照组（0.69 ± 0.18），差异均有统计学意义（t =
6.314，P ＜ 0.001；t = 10.081，P ＜ 0.001）。重度CHB组miR-155相对表达量为0.52 ± 0.13，显著低
于中度CHB组（0.65 ± 0.16）和轻度CHB组（0.73 ± 0.20）；SOCS1 mRNA相对表达量为1.50 ± 0.25，显著
高于中度CHB组（1.21 ± 0.33）和轻度CHB组（1.09 ± 0.31），差异均有统计学意义（P均 ＜ 0.05）。CHB患者
miR-155和SOCS1 mRNA表达水平呈显著负相关（r = -0.695，P ＜ 0.01）。重度CHB组血清ALT、AST、TBil
水平分别为（95.97 ± 11.98）U/L、（75.93 ± 10.27）U/L、（22.21 ± 4.09）μmol/L，均显著高于轻度CHB组
[（79.73 ± 10.86）U/L、（61.57 ± 9.16）U/L、（18.06 ± 3.14）μmol/L]和中度CHB组[（86.05 ± 12.14）、
（66.66 ± 9.38）U/L、（19.73 ± 3.52）μmol/L]，差异有统计学意义（P均 ＜ 0.05），中度CHB组血清
ALT、AST、TBil水平与轻度CHB组相比，差异无统计学意义（P均 ＞ 0.05）。CHB患者miR-155相对
表达水平与ALT、AST、TBil水平呈负相关（r = -0.457、-0.531、-0.389，P均 ＜ 0.05），SOCS1 mRNA
与ALT、AST、TBil水平呈正相关（r = 0.419、0.520、0.371，P均 ＜ 0.05）。结论 CHB患者PBMC中
miR-155和SOCS1 mRNA的异常表达与肝功能损伤程度密切相关。
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Abstract: Objective To analyze the expression levels of miR-155 and suppressor of cytokine signaling 1 (SOCS1)
mRNA in peripheral blood mononuclear cells (PBMC) of patients with chronic hepatitis B (CHB), and to explore their
relationships with the degree of liver injury. Methods A total of 95 patients with CHB in Nanjing Hospital Affiliated
to Nanjing University of Traditional Chinese Medicine from March 2016 to June 2018 were continuously enrolled
as CHB group. The patients were furtherly divided into mild CHB group (31 cases), moderate CHB group (38 cases)
and severe CHB group (26 cases) according to the results of liver histopathology. Another 50 healthy persons were
selected as control group during the same period. The relative expressions of miR-155 and SOCS1 mRNA in PBMC
were detected by RT-PCR, serum liver function indicators [alanine transaminase (ALT), aspartate aminotransferase
(AST), total bilirubin (TBil)] were detected by ELISA. The relationships of the expressions of miR-155 and SOCS1 mRNA
in PBMC with the clinical grading and liver function indexes in patients with CHB were analyzed. Results The miR-155
relative expression of patients in CHB group was significantly lower than that in control group [(0.64 ± 0.15) vs (0.86 ± 0.27)],
and the relative expression of SOCS1 mRNA was significantly higher than that in control group [(1.25 ± 0.37)
vs (0.69 ± 0.18)], the differences were statistically significant (t = 6.314, P ＜ 0.001; t = 10.081, P ＜ 0.001). The miR-155
relative expression of patients in severe CHB group was 0.52 ± 0.13, which was lower than those in moderate CHB
group (0.65 ± 0.16) and mild CHB group (0.73 ± 0.20), and the relative expression of SOCS1 mRNA was 1.50 ± 0.25,
which was higher than those in moderate CHB group (1.21 ± 0.33) and mild CHB group (1.09 ± 0.31), the differences
were statistically significant (P ＜ 0.05). There was a significant negative correlation between the expressions of
miR-155 and SOCS1 mRNA in CHB group (r = -0.695, P ＜ 0.01). The levels of serum ALT, AST, and TBil of
patients in severe CHB group [(95.97 ± 11.98) U/L, (75.93 ± 10.27) U/L, (22.21 ± 4.09) μmol/L)] were higher
than those in mild CHB group [(79.73 ± 10.86) U/L, (61.57 ± 9.16) U/L, (18.06 ± 3.14) μmol/L] and moderate
CHB group [(86.05 ± 12.14) U/L, (66.66 ± 9.38) U/L, (19.73 ± 3.52) μmol/L], the differences were statistically
significant (P ＜ 0.05); there were no significant differences in serum ALT, AST and TBil levels of patients between
moderate CHB group and mild CHB group (P ＞ 0.05). The miR-155 expression level of patients in CHB group
was negatively correlated with ALT, AST and TBil levels (r=-0.457, -0.531, -0.389, all P ＜ 0.05), SOCS1 mRNA
was positively correlated with ALT, AST, and TBil levels (r = 0.419, 0.520, 0.371, all P ＜ 0.05). Conclusion Abnormal
expression of miR-155 and SOCS1 mRNA in PBMC of patients with CHB is closely related to the degree of liver injury.
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