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Abstract: Objective To investigate the differential expression of carcinoma-related genes
in peripheral blood leukocytes of patients with chronic hepetitis B (CHB), hepatitis B virus
(HBV) carriers, patients with HBV-related liver failure and healthy controls. Methods Patients
with CHB (CHB group, 51 cases), HBV carriers (ASC group, 51 cases), patients with HBVrelated liver failure (LF group, 15 cases) and healthy controls (HC group, 51 cases) in the
Second People’s Hospital of Yunnan Province from February 2019 to September 2019 were
enrolled. Peripheral blood was collected from all study subjects and total RNA was extracted
from peripheral blood leukocytes. The relative expression levels of DNAJC6, GSE1, HIPK3,
MBOAT2, UBXN7 linear and circular RNA were detected by quantitative reverse transcriptasemediated polymerase chain reaction (qRT-PCR), and the changes of circRNA/linear RNA
expression ratio among groups were evaluated. Results Compared with healthy controls, the
relative expression levels of Lin-DNAJC6 (3.59 ± 1.47 vs 2.36 ± 1.10), Lin-HIPK3 (1.79 ± 0.71
vs 1.33 ± 0.65), Lin-UBXN7 (8.68 ± 1.96 vs 3.36 ± 0.86), Circ-GSE1 (2.71 ± 1.47 vs 1.83 ± 1.09)
and Circ-UBXN7 (2.88 ± 1.37 vs 2.03 ± 1.19) were signifcant higher in ASC group, while the
relative expression levels of Lin-GSE1 (1.65 ± 0.94 vs 2.04 ± 0.79) and Circ-HIPK3 (1.93 ± 1.09
vs 3.85 ± 1.23) were signifcantly lower (all P ＜ 0.05). Compared with healthy controls, the
relative expression levels of Lin-DNAJC6 (6.73 ± 1.48 vs 2.36 ± 1.10), Lin-HIPK3 (7.24 ± 0.85
vs 1.33 ± 0.65), Lin-MBOAT2 (6.58 ± 0.72 vs 1.35 ± 0.70), Circ-DNAJC6 (5.26 ± 1.86 vs
3.41 ± 1.75), Circ-GSE1 (2.61 ± 1.68 vs 1.83 ± 1.09), Circ-MBOAT2 (5.50 ± 1.82 vs 3.22 ± 1.52)
and Circ-UBXN7 (4.61 ± 1.36 vs 2.03 ± 1.19) in CHB group were signifcantly higher, while
the relative expression level of Lin-UBXN7 (2.47 ± 0.83 vs 3.36 ± 0.86) was significantly
lower (all P ＜ 0.05). Compared with healthy controls, the relative expression levels of LinDNAJC6 (5.10 ± 2.49 vs 2.36 ± 1.10), Lin-HIPK3 (2.47 ± 1.22 vs 1.33 ± 0.65) and CircGSE1 (2.98 ± 2.12 vs 1.83 ± 1.09) were signifcantly higher in LF group, while the relative
expression levels of Circ-HIPK3 (2.29 ± 1.40 vs 3.85 ± 1.23) was significantly lower (all
P ＜ 0.05). Meanwhile, the circRNA /linear RNA expression ratio of DNAJC6, GSE1, HIPK3,
MBOAT2, UBXN7 were different in each group. The circRNA/linear RNA expression ratio
of GSE1 (median: 1.77 vs 0.93) in ASC group were significantly higher than HC group
(P ＜ 0.05). The circRNA /linear RNA expression ratio of DNAJC6 (median: 1.10 vs 1.52),
HIPK3 (median: 1.05 vs 2.95) and UBXN7 (median: 0.31 vs 0.56) in ASC group were
signifcantly lower than HC group (all P ＜ 0.05). The circRNA /linear RNA expression ratio
of GSE1 (median: 1.43 vs 0.93) and UBXN7 (median: 1.82 vs 0.56) in patients with CHB
were signifcantly higher than those of healthy controls (all P ＜ 0.05). The circRNA /linear
RNA expression ratio of DNAJC6 (median: 0.78 vs 1.52), HIPK3 (madian: 0.50 vs 2.95) and
MBOAT2 (median: 0.82 vs 2.14) in patients with CHB were significantly lower than those
of healthy controls (all P ＜ 0.05). The circRNA /linear RNA expression ratio of DNAJC6
(median: 0.65 vs 1.52) of patients in LF group were signifcantly lower than those of healthy
controls (P ＜ 0.05). Conclusions DNAJC6, GSE1, HIPK3, MBOAT2, UBXN7 linear RNA
and circRNA were differentially expressed in peripheral blood leukocytes of patients with
HBV-infected liver disease, suggesting that circRNA may be closely related to the progression
of liver disease associated with HBV infection.
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