中国肝脏病杂志(电子版)

位置：首页 >> 期刊文章

CT动态观察不同剂量高脂饵料复制非酒精性脂肪肝动物模型效果

作者：王记红胡小林高玉玖朱德文任永生谭华炳

单位：1.湖北医药学院第三临床医学院十堰市 442000 2.湖北医药学院附属人民医院生理教研室十堰市 442000 3.湖北医药学院附属人民医院感染性疾病科十堰市 442000

关键词：高脂饲料剂量控制 CT监测非酒精性脂肪性肝病

分类号：

出版年,卷(期):页码：2011,3(3):1-4

摘要：

摘要：目的探讨CT动态观察不同剂量高脂饵料复制非酒精性脂肪性肝病（NAFLD）动物模型的可行性。方法选择40只日本大耳白兔随机分为重度NAFLD模型组（高剂量组）、轻度NAFLD模型组（低剂量组）及对照组。高剂量组给予高脂饲料160 g/（兔•d），低剂量组给予高脂饲料80 g/（兔•d）＋普通饲料80 g/（兔•d），对照组给予普通饲料160 g/（兔•d）。通过CT观察各组脂肪肝程度。实验结束后处死动物，留取血和肝脏组织备用。检测血浆和肝组织甘油三酯（TG）、胆固醇（TC）。肝组织进行HE染色并进行观察。结果高剂量组TC和TG分别为（32.12 ± 1.25）mmol/L和（6.02 ± 2.12）mmol/L，低剂量组分别为（18.34 ± 2.10）mmol/L和（4.39 ± 1.93）mmol/L，与对照组比较差异均有统计学意义（P ＜ 0.01）。高剂量组TG和TC高于低剂量组，差异有统计学意义（P＜ 0.01）。高剂量组、低剂量组和对照组的肝组织TG分别为（0.71 ± 0.07）mmol/L、（0.52 ± 0.08）mmol/L、（0.29 ± 0.10）mmol/L，差异有显著统计学意义（P ＜ 0.01）。高剂量组肝组织TG与低剂量组比较差异有显著统计学意义（P ＜ 0.01）。高剂量组、低剂量组和对照组肝脏CT值分别为（31.3 ± 2.4）HU、（42.1 ± 3.5）HU、（58.9 ± 1.9）HU，差异有显著统计学意义（P ＜ 0.01）。高剂量组肝脏CT值与低剂量组比较差异有显著统计学意义（P ＜ 0.01）。高剂量组肝脏病理学检测结果为重度NAFLD，低剂量组为轻度～中度NAFLD，对照组为正常肝脏组织。结论高脂饲料剂量控制联合CT动态观察可以建立不同程度的兔NAFLD模型。

Abstract: Objective To explore the feasibility of dynamic observation on the effect of NAFLD animal model induced by different doses of high-fat baits through CT. Methods Total of 40 Japanese white rabbits were randomly divided into severe group with NAFLD (high dose group), mild group with NAFLD (low dose group), blank control group (blank group). High dose group were given high fat diet with 160 g/(rabbit•day), low dose group were given high fat diet and normal diet with 80 g/(rabbit•day), respectively, blank group were given normal feed with 160 g/(rabbit•day). CT values were detected in order to observe the degree of fatty liver of the high dose group. Animals were executed after the experiment and the blood and liver tissue were reserved. Triglyceride (TG), cholesterol (TC) of plasma and liver tissue were also detected. Liver tissues were detected with HE staining to observe pathological changes. Results TC and TG level of high dose group were (32.12 ± 1.25) mmol/L and (6.02 ± 2.12) mmol/L, respectively, which were (18.34 ± 2.10) mmol/L and (4.39 ± 1.93) mmol/L of low dose group, the differences were both significant compared with blank group (P ＜ 0.01). TG and TC of high dose group were higher than that in low dose group, with the difference significant (P ＜ 0.01). TG levels of liver tissue in high dose group, low dose group blank group were (0.71 ± 0.07) mmol/L, (0.52 ± 0.08) mmol/L and (0.29 ± 0.10) mmol/L, respectively, with a significant difference among the three groups (P ＜ 0.01). Also the difference was significant between high dose group and low dose group (P ＜ 0.01). Also the difference was significant between high dose group and low dose group (P ＜ 0.01). CT values of high dose group and mild group were (31.3 ± 2.4) HU and (42.1 ± 3.5) HU, respectively, both with significant differences compared with blank group (58.9 ± 1.9) HU (P ＜ 0.01). Also the difference between high dose group and low dose group was significant (P ＜ 0.01). Liver pathology in high dose group was detected as severe NAFLD and mild to moderate NAFLD in low dose group, which was normal in blank group. Conclusions Different doses of high-fat baits and dynamic observation with CT can establish different degree of NAFLD rabbit model.

基金项目：

湖北省教育厅科技项目（D20102104）；湖北省卫生厅科研项目（JX5B75）；十堰市科技局科技项目（2010st33）；2010年湖北医药学院学生科研项目(2010XSA19)

作者简介：

参考文献：

服务与反馈：

【文章下载】【加入收藏】

buy biaxin pills

get cleocin

buy dexone online safe