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抑制素在人肝癌细胞SMMC-7721和Huh-7中的表达及其编码序列扩增

作者：党双锁孙明珠寻萌杨娥李亚萍王秀芳王文俊李梅

单位：西安交通大学医学院第二附属医院感染科西安 710004 西安交通大学医学院免疫与病原生物学系西安 710061

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出版年,卷(期):页码：2010,2(3):1-5

摘要：

目的检测抑制素在人肝癌细胞SMMC7721及Huh-7中的基因表达，扩增抑制素全长编码序列。方法分别提取肝癌细胞SMMC7721及Huh-7细胞总RNA，逆转录合成cDNA。使用Real time PCR，以GAPDH为内参检测抑制素的mRNA表达情况。采用RT-PCR方法扩增抑制素全长编码序列。结果 Real time PCR结果显示，SMMC7721和Huh-7肝癌细胞株中均有抑制素的表达，且SMMC7721细胞中抑制素表达量是Huh-7细胞中的6.652倍。RT-PCR方法扩增得到约835 bp的抑制素全长编码序列，条带特异，无非特异性扩增。结论人肝癌细胞株SMMC7721中抑制素的表达量较高。

Objective To detect gene expression of prohibitin in human liver cancer cells SMMC7721 and Huh-7, and amplify the full coding sequence of prohibitin. Methods The total cellular RNA of SMMC7721 and Huh-7 were extracted, then the first strand cDNA was synthesized by reverse transcription. The expression of prohibitin mRNA was verified by Real time PCR, with GAPDH as an internal validation. RT-PCR was used to amplify the full coding sequence of prohibitin. Results The results of Real time PCR showed prohibitin was expressed in both SMMC7721 and Huh-7 cells. And the expression level of prohibitin in SMMC7721 cells was 6.652 times higher than that in Huh-7 cells. About 835 bp full coding sequence of prohibitin was successfully amplified by RT-PCR, without unspecific amplificaiton. Conclusions The expression level of prohibitin in human liver cancer cells SMMC7721 was relatively high.

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