Abstract: Objective To investigate the change and clinical significance of combined detection of bile acid (TBA), prealbumin (PA), total glutamate homocysteine proteaseInhibitors C (Cys C), dehydrogenase (GLDH) and cholinesterase (CHE) in patients with liver disease and to provide the basis for the diagnosis and treatment. Methods Total of 253 patients with liver disease were divided into 4 groups: patients with acute hepatitis (84 cases), chronic hepatitis (93 cases), liver cirrhosis (46 cases) and liver cancer (30 cases); 56 cases of healthy persons were taken as the control group. Serum concentration of TBA, PA, Cys C, GDLH and CHE were detected and analyzed, respectively. Results The concentration of PA and CHE reduced signifi cantly in all patients with liver disease, which were more obvious in patients with liver cirrhosis and liver cancer; TBA, Cys C and GLDH were signifi cantly increased, following by liver cirrhosis group, chronic hepatitis group and acute hepatitis group, which were statistically signifififi cant compared with the control group (P ＜ 0.05). Conclusions Variation on concentration of PA and CHE can refl ect the damage degree of liver cells in early stage of hepatitis. TBA, Cys C and GLDH are sensitive indexes of all sorts of liver disease, which has important clinical signifi cance for early diagnosis and prognosis of hepatitis.