Abstract: Objective To evaluate the effects of strong low-frequency sound on liver and spleen of rats. Methods The rats were randomly exposed to a closed sound field and acousticly stimulated by strong low-frequency sound of 103 Hz or 300 Hz, respectively. The exposure time was 5 min and 10 min, respectively. On the basis of observation time, each group were divided into immediate group, 3 days group and 7 days group. The liver and spleen histopathology were observed based on the injury severity criteria. Results Different degrees of vascular dilation, congestion, edema were found in liver and spleen of the exposed rats when observed under light microscope. Conclusions Strong low-frequency sound can cause liver and spleen injury.