Abstract: Objective To explore the relationship between the expression of TGF-β,17 in serum and hepatitis B disease progression. Methods We used ELISA method to detect the serum level of TGF-β, IL-17 in epatient, which were choosed from our hospital from 2010 June to 2012 December and were diagosised hepatitis B by the liver tissue pathology biopsy, then to analysis the relationship of between inflammatory activity, fibrosis degree and TGF-β IL-17 level. Results The TGF-β, IL-17 serum levels have significant difference among different degree of inflammatory activity, being analysised by Bonferroni method (P ＜ 0.05). There are positive correlation between the serum levels and grade of inflammation by Sypearman rank correlation analysis (r = 0.517, P = 0.004; r = 0.747, P = 0.002). The TGF-β, IL-17 serum levels have significant difference among different degree of fibrosis group by Bonferroni methodhas (P ＜ 0.05).are positive correlatioship between TGF-β serum level and the different degree of fibrosis by Spearman rank correlation analysis (r = 0.677, P = 0.010). There are significant correlationship between the serum expression of IL-17 and TGF-β in chronic hepatitis B patients (r = 0.683, P = 0.010). Conclusions The high expression level of TGF-β IL-17 in peripheral blood in patients with hepatitis B participate in the activity of inflammation reaction and fibrosis, TGF-β play an important role in liver fibrosis.