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清热解毒化浊片对脂肪性肝病大鼠脂肪因子ADP及炎性因子TNF-α、IL-10的影响

作者：毛娅男赵国荣陈研焰戴玉微崔玉晖

单位：湖南中医药大学中医学院湖南长沙 410208

关键词：清热解毒化浊片脂肪性肝病 ADP TNF-α IL-10

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出版年,卷(期):页码：2016,8(1):67-71

摘要：

摘要：目的研究清热解毒化浊片对脂肪性肝病大鼠脂肪因子ADP及炎性因子TNF-α、IL-10的影响。
方法采用高脂饮食、酒精灌胃建立脂肪性肝病大鼠模型。造模成功后，随机分为清热解毒化浊片
组、非诺贝特组、模型组和正常组4组，每组10只，清热解毒化浊片组按0.081 g/ml灌胃，非诺贝特
组按0.083 g/ml灌胃，模型组及正常组予等体积蒸馏水灌胃，每组动物灌胃量均为1 ml/（100 g·d）。
经治疗30天后观察肝组织病理学改变，采用全自动生化仪检测血清甘油三酯（TG）、总胆固醇
（TC）、丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）活性，ELISA法检测血清脂
联素（ADP）、白细胞介素10（IL-10）、肿瘤坏死因子α（TNF-α）的表达，免疫组织化学二步法测
定IL-10、TNF-α在肝组织中的表达。结果光学显微镜下观察，清热解毒化浊片组大鼠肝组织脂肪沉
积、炎性浸润情况明显改善。与正常组比较，模型组血清TG、TCHO、ALT、AST、TNF-α显著升高
（P值分别为0.000、0.006、0.005、0.005、0.000），血清ADP显著降低（P = 0.005），肝组织TNF-α
显著升高（P = 0.000）。与模型组比较，清热解毒化浊片组血清TG、TCHO、ALT、AST、TNF-α均
显著降低（P均为0.000），血清ADP、IL-10显著升高（P = 0.02、0.000)，肝组织TNF-α显著降低（P =
0.000），肝组织IL-10显著升高（P = 0.000）。结论清热解毒化浊片可调节肝细胞脂质代谢，减少炎
性损伤，其作用机制可能是通过促进脂肪因子ADP的释放，减少脂质堆积，促进抑炎因子IL-10的表
达，降低促炎因子TNF-α的释放，从而达到降脂抗炎，保肝护肝的目的。

Abstract: Objective To investigate the effects of Qingre Jiedu Huazhuo tablets on ADP, TNF-α and IL-10 in
rat model of fatty liver disease. Methods The rat liver inflammation model was established by feeding high
sugar, fat and liquor. The rats were randomly divided into normal group, model group, Fenofibrate group and
Qingre Jiedu Huazhuo group, 10 rats in each group. Qingre Jiedu Huazhuo tablets (0.081 g/ml) and Fenofibrate
(0.083 g/ml) were used to treat liver inflammatory model rat for 30 days [1 ml/(100g·d)], respectively. The
content of blood serum alanine transaminase (ALT), aspartate transaminase (AST), triglyceride (TG) and total
cholesterin (TC) were measured by colorimetry; tumor necrosis factor-α (TNF-α), interleukin-10 (IL-10) and
adiponectin (ADP) were measured by enzyme-linked immumsorbent assay (ELISA). Pathologic changes of
liver were measured by a microscope, and the two-step method of immunohistochemistry (IH) was adopted
to test the expression of TNF-α and IL-10 in live tissues. Results Light microscopic inspection: Qingre Jiedu
Huazhuo tablets can suppress liver inflammation and lipid deposition. The expression of serum TG, TCHO,
ALT, AST and TNF-α in model group were significantly higher than those in normal control group (P = 0.000,
0.006, 0.005, 0.005, 0.000, respectively). The expression of ADP in model group was significantly lower
than that in normal group (P = 0.005). Compared with the model group, the expression of TG, TCHO, ALT,
AST and TNF-α were significantly lower in Qingre Jiedu Huazhuo tablets group (P = 0.000). The expression
of ADP and IL-10 were significantly higher in Qingre Jiedu Huazhuo tablets group (P = 0.02, 0.000). Qingre
Jiedu Huazhuo tablets can significantly reduce the level of TNF-α (P = 0.000) and increase the level of IL-10
(P = 0.000). Conclusions Qingre Jiedu Huazhuo tablets can regulate lipid metabolism and resist inflammatory
injury, which may be associated with the reduced release of promoting inflammatory cytokines TNF-α and the
promoting of the expression of anti-inflammatory cytokine IL-10 and adipokine ADP.

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